Chitosan, Carbon Quantum Dot, and Silica Nanoparticle Mediated dsRNA Delivery for Gene Silencing in Aedes aegypti: A Comparative Analysis : A Comparative Analysis

TitleChitosan, Carbon Quantum Dot, and Silica Nanoparticle Mediated dsRNA Delivery for Gene Silencing in Aedes aegypti: A Comparative Analysis : A Comparative Analysis
Publication TypeJournal Article
Year of Publication2015
AuthorsDas, S, Debnath, N, Cui, Y, Unrine, J, Palli, SReddy
JournalACS Applied Materials & Interfaces
Volume7
Issue35
Pagination19530 - 19535
Date Published09/2015
ISSN1944-8244
Abstract

In spite of devastating impact of mosquito borne pathogens on humans, widespread resistance to chemical insecticides and environmental concerns from residual toxicity limit mosquito control strategies. We tested three nanoparticles, chitosan, carbon quantum dot (CQD), and silica complexed with dsRNA, to target two mosquito genes (SNF7 and SRC) for controlling Aedes aegypti larvae. Relative mRNA levels were quantified using qRT-PCR to evaluate knockdown efficiency in nanoparticle-dsRNA treated larvae. The knockdown efficiency of target genes correlated with dsRNA mediated larval mortality. Among the three nanoparticles tested, CQD was the most efficient carrier for dsRNA retention, delivery, and thereby causing gene silencing and mortality in Ae. aegypti.

URLhttp://pubs.acs.org/doi/10.1021/acsami.5b05232http://pubs.acs.org/doi/pdf/10.1021/acsami.5b05232
DOI10.1021/acsami.5b05232
Short TitleACS Appl. Mater. Interfaces