At the first step, a calibration curve must be created to measure fullerene concentrations. Fullerenes must be suspended in an organic solvent (toluene in this case) that will be used as the carrier fluid through the instrument column. Fullerene standards was prepared by adding different amounts of fullerene standard into 10 ml of toluene and sonicating for 1 h. Standards
were stored in amber vials at 4°C and allowed to come to room temperature before use. As shown in Figure 1, calibration curve for C60 ranged from 15 ppb to 15 ppm.