CeO2 nanoparticles induce DNA damage towards human dermal fibroblasts in vitro

TitleCeO2 nanoparticles induce DNA damage towards human dermal fibroblasts in vitro
Publication TypeJournal Article
Year of Publication2009
AuthorsAuffan M, Rose J, Orsiere T, De Meo M, Thill A, Zeyons O, Proux O, Masion A, Chaurand P, Spalla O, Botta A, Wiesner MR, Bottero J-Y
JournalNanotoxicology
Volume3
Issue2
Pagination161 - 171
Date Published01/2009
ISSN1743-5404
Abstract

Cerium dioxide nanoparticles have been proposed for an increasing number of applications in biomedicine, cosmetic, as polishing materials and also as byproducts from automotive fuel additives. The aim of this study was to examine the potential in vitro cyto- and genotoxicity of nano-sized CeO2 (7 nm) on human dermal fibroblasts. By combining a physico-chemical and a (geno)toxicological approach, we defined the causal mechanisms linking the physico-chemical properties of nano-CeO2 with their biological effects. Using X-ray absorption spectroscopy, we observed a reduction of 21±4% of the Ce4+ atoms localized at the surface of CeO2 nanoparticles due to the interactions with organic molecules present in biological media. These particles induced strong DNA lesions and chromosome damage related to an oxidative stress. These genotoxic effects occurred at very low doses, which highlighted the importance of a genotoxicological approach during the assessment of the toxicity of nanoparticles.

DOI10.1080/17435390902788086
Short TitleNanotoxicology